› 1bx6

X-ray diffraction
2.1Å resolution

CRYSTAL STRUCTURE OF THE POTENT NATURAL PRODUCT INHIBITOR BALANOL IN COMPLEX WITH THE CATALYTIC SUBUNIT OF CAMP-DEPENDENT PROTEIN KINASE

Released:
DOI: 10.2210/pdb1bx6/pdb
PDB entry 1bx6 coloured by chain, front view.
PDB entry 1bx6 coloured by chain, side view.
PDB entry 1bx6 coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Crystal structure of the potent natural product inhibitor balanol in complex with the catalytic subunit of cAMP-dependent protein kinase.
Narayana N, Diller TC, Koide K, Bunnage ME, Nicolaou KC, Brunton LL, Xuong NH, Ten Eyck LF, Taylor SS
Biochemistry 38 2367-76 (1999)
PMID: 10029530

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-138536 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
cAMP-dependent protein kinase catalytic subunit alpha Chain: A
Molecule details ›
Chain: A
Length: 350 amino acids
Theoretical weight: 40.74 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: P05132 (Residues: 2-351; Coverage: 100%)
Gene names: Pkaca, Prkaca
Sequence domains: Protein kinase domain
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand BA1 1 x BA1
2 modified residues:
Ligand SEP 2 x SEP
Ligand TPO 1 x TPO

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RUH2R
Spacegroup: P212121
Unit cell:
a: 57.19Å b: 73.18Å c: 99.77Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.21 0.202 0.34
Expression system: Escherichia coli

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Citations

6 review citations

Protein kinase C: poised to signal.
Newton AC. (2010)
5 more

20 mentions without citation

Solution NMR Spectroscopy for the Study of Enzyme Allostery.
Lisi et al. (2016)
19 more