› 1bvy

X-ray diffraction
2.03Å resolution

COMPLEX OF THE HEME AND FMN-BINDING DOMAINS OF THE CYTOCHROME P450(BM-3)

Released:
DOI: 10.2210/pdb1bvy/pdb
PDB entry 1bvy coloured by chain, front view.
PDB entry 1bvy coloured by chain, side view.
PDB entry 1bvy coloured by chain, top view.
Source organism: Priestia megaterium
Primary publication:
Structure of a cytochrome P450-redox partner electron-transfer complex.
Sevrioukova IF, Li H, Zhang H, Peterson JA, Poulos TL
Proc Natl Acad Sci U S A 96 1863-8 (1999)
PMID: 10051560

Function and Biology Details

Reactions catalysed: 
RH + [reduced NADPH--hemoprotein reductase] + O(2) = ROH + [oxidized NADPH--hemoprotein reductase] + H(2)O
NADPH + n oxidized hemoprotein = NADP(+) + n reduced hemoprotein
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-147080 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Bifunctional cytochrome P450/NADPH--P450 reductase Chains: A, B
Molecule details ›
Chains: A, B
Length: 458 amino acids
Theoretical weight: 52.4 KDa
Source organism: Priestia megaterium
Expression system: Escherichia coli
UniProt:
  • Canonical: P14779 (Residues: 2-459; Coverage: 44%)
Gene names: BG04_163, cyp102, cyp102A1
Sequence domains: Cytochrome P450
Structure domains: Cytochrome P450
Bifunctional cytochrome P450/NADPH--P450 reductase Chain: F
Molecule details ›
Chain: F
Length: 191 amino acids
Theoretical weight: 20.8 KDa
Source organism: Priestia megaterium
Expression system: Escherichia coli
UniProt:
  • Canonical: P14779 (Residues: 460-650; Coverage: 18%)
Gene names: BG04_163, cyp102, cyp102A1
Sequence domains: Flavodoxin
Structure domains: Rossmann fold

Ligands and Environments


Cofactor: Ligand HEM 2 x HEM

Cofactor: Ligand FMN 1 x FMN
1 bound ligand:
Ligand EDO 5 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL7-1
Spacegroup: P212121
Unit cell:
a: 58.84Å b: 94.68Å c: 209.06Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.187 not available 0.275
Expression system: Escherichia coli

Quick links

Citations

52 review citations

The molecular biology, biochemistry, and physiology of human steroidogenesis and its disorders.
Miller et al. (2011)
51 more

29 mentions without citation

Oxygen Activation and Radical Transformations in Heme Proteins and Metalloporphyrins.
Huang et al. (2018)
28 more