› 1bvv

X-ray diffraction
1.8Å resolution

SUGAR RING DISTORTION IN THE GLYCOSYL-ENZYME INTERMEDIATE OF A FAMILY G/11 XYLANASE

Released:
DOI: 10.2210/pdb1bvv/pdb
PDB entry 1bvv coloured by chain, front view.
PDB entry 1bvv coloured by chain, side view.
PDB entry 1bvv coloured by chain, top view.
Source organism: Niallia circulans
Primary publication:
Sugar ring distortion in the glycosyl-enzyme intermediate of a family G/11 xylanase.
Sidhu G, Withers SG, Nguyen NT, McIntosh LP, Ziser L, Brayer GD
Biochemistry 38 5346-54 (1999)
PMID: 10220321

Function and Biology Details

Reaction catalysed: 
Endohydrolysis of (1->4)-beta-D-xylosidic linkages in xylans
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-140770 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Endo-1,4-beta-xylanase Chain: A
Molecule details ›
Chain: A
Length: 185 amino acids
Theoretical weight: 20.41 KDa
Source organism: Niallia circulans
Expression system: Escherichia coli
UniProt:
  • Canonical: P09850 (Residues: 29-213; Coverage: 100%)
Gene name: xlnA
Sequence domains: Glycosyl hydrolases family 11
Structure domains: Jelly Rolls

Ligands and Environments

Carbohydrate polymer : NEW Components: DFX, XYP
Carbohydrate polymer
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RUH3R
Spacegroup: P212121
Unit cell:
a: 43.87Å b: 52.79Å c: 79.09Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.189 0.189 not available
Expression system: Escherichia coli

Quick links

Citations

9 review citations

Glycosidase mechanisms.
Rye et al. (2000)
8 more

11 mentions without citation

Protonation and pK changes in protein-ligand binding.
Onufriev et al. (2013)
10 more