› 1brn

X-ray diffraction
1.76Å resolution

SUBSITE BINDING IN AN RNASE: STRUCTURE OF A BARNASE-TETRANUCLEOTIDE COMPLEX AT 1.76 ANGSTROMS RESOLUTION

Released:
DOI: 10.2210/pdb1brn/pdb
PDB entry 1brn coloured by chain, front view.
PDB entry 1brn coloured by chain, side view.
PDB entry 1brn coloured by chain, top view.
Source organism: Bacillus amyloliquefaciens
Primary publication:
Subsite binding in an RNase: structure of a barnase-tetranucleotide complex at 1.76-A resolution.
Buckle AM, Fersht AR
Biochemistry 33 1644-53 (1994)
PMID: 8110767

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-113507 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
Ribonuclease Chains: L, M
Molecule details ›
Chains: L, M
Length: 110 amino acids
Theoretical weight: 12.4 KDa
Source organism: Bacillus amyloliquefaciens
Expression system: Escherichia coli
UniProt:
  • Canonical: P00648 (Residues: 48-157; Coverage: 89%)
Sequence domains: ribonuclease
Structure domains: Microbial ribonucleases
DNA (5'-D(*CP*GP*AP*C)-3') Chains: A, B
Molecule details ›
Chains: A, B
Length: 4 nucleotides
Theoretical weight: 1.18 KDa
Source organism: Bacillus amyloliquefaciens
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: P1
Unit cell:
a: 39.866Å b: 54.286Å c: 29.607Å
α: 107.77° β: 89.05° γ: 96.37°
R-values:
R R work R free
0.19 0.19 not available
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

3 review citations

Nucleases: diversity of structure, function and mechanism.
Yang W. (2011)
2 more

11 mentions without citation

3D domain swapping: as domains continue to swap.
Liu et al. (2002)
10 more