› 1bo1

X-ray diffraction
3Å resolution

PHOSPHATIDYLINOSITOL PHOSPHATE KINASE TYPE II BETA

Released:
DOI: 10.2210/pdb1bo1/pdb
PDB entry 1bo1 coloured by chain, front view.
PDB entry 1bo1 coloured by chain, side view.
PDB entry 1bo1 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structure of type IIbeta phosphatidylinositol phosphate kinase: a protein kinase fold flattened for interfacial phosphorylation.
Rao VD, Misra S, Boronenkov IV, Anderson RA, Hurley JH
Cell 94 829-39 (1998)
PMID: 9753329

Function and Biology Details

Reaction catalysed: 
ATP + 1-phosphatidyl-1D-myo-inositol 5-phosphate = ADP + 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-160254 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Phosphatidylinositol 5-phosphate 4-kinase type-2 beta Chains: A, B
Molecule details ›
Chains: A, B
Length: 416 amino acids
Theoretical weight: 47.45 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P78356 (Residues: 1-416; Coverage: 100%)
Gene names: PIP4K2B, PIP5K2B
Sequence domains: Phosphatidylinositol-4-phosphate 5-Kinase
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X9B
Spacegroup: C2221
Unit cell:
a: 109.915Å b: 182.403Å c: 106.472Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.229 0.229 0.299
Expression system: Escherichia coli

Quick links

Citations

41 review citations

Phosphoinositides: tiny lipids with giant impact on cell regulation.
Balla T. (2013)
40 more

19 mentions without citation

Advances in chemical proteomic evaluation of lipid kinases-DAG kinases as a case study.
Ware et al. (2021)
18 more