› 1bi5

X-ray diffraction
1.56Å resolution

CHALCONE SYNTHASE FROM ALFALFA

Released:
DOI: 10.2210/pdb1bi5/pdb
PDB entry 1bi5 coloured by chain, front view.
PDB entry 1bi5 coloured by chain, side view.
PDB entry 1bi5 coloured by chain, top view.
Source organism: Medicago sativa
Primary publication:
Structure of chalcone synthase and the molecular basis of plant polyketide biosynthesis.
Ferrer JL, Jez JM, Bowman ME, Dixon RA, Noel JP
Nat Struct Biol 6 775-84 (1999)
PMID: 10426957

Function and Biology Details

Reaction catalysed: 
3 malonyl-CoA + 4-coumaroyl-CoA = 4 CoA + naringenin chalcone + 3 CO(2)
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-151628 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Chalcone synthase 2 Chain: A
Molecule details ›
Chain: A
Length: 389 amino acids
Theoretical weight: 42.79 KDa
Source organism: Medicago sativa
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P30074 (Residues: 1-389; Coverage: 100%)
Gene name: CHS2
Sequence domains:
Structure domains: Peroxisomal Thiolase; Chain A, domain 1

Ligands and Environments

No bound ligands
1 modified residue:
Ligand CSD 1 x CSD

Experiments and Validation Details

Entry percentile scores
X-ray source: MACSCIENCE
Spacegroup: P3221
Unit cell:
a: 97.54Å b: 97.54Å c: 65.52Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.132 not available 0.193
Expression system: Escherichia coli BL21

Quick links

Citations

52 review citations

Biosynthesis of flavonoids and effects of stress.
Winkel-Shirley B. (2002)
51 more

16 mentions without citation

Structure and function of enzymes involved in the biosynthesis of phenylpropanoids.
Ferrer et al. (2008)
15 more