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X-ray diffraction
2.5Å resolution

ALPHA-AMYLASE FROM BACILLUS SUBTILIS COMPLEXED WITH MALTOPENTAOSE

Released:
DOI: 10.2210/pdb1bag/pdb
PDB entry 1bag coloured by chain, front view.
PDB entry 1bag coloured by chain, side view.
PDB entry 1bag coloured by chain, top view.
Source organism: Bacillus subtilis
Primary publication:
Crystal structure of a catalytic-site mutant alpha-amylase from Bacillus subtilis complexed with maltopentaose.
Fujimoto Z, Takase K, Doui N, Momma M, Matsumoto T, Mizuno H
J Mol Biol 277 393-407 (1998)
PMID: 9514750

Function and Biology Details

Reaction catalysed: 
Endohydrolysis of (1->4)-alpha-D-glucosidic linkages in polysaccharides containing three or more (1->4)-alpha-linked D-glucose units
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132828 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Alpha-amylase Chain: A
Molecule details ›
Chain: A
Length: 425 amino acids
Theoretical weight: 47.07 KDa
Source organism: Bacillus subtilis
Expression system: Bacillus subtilis
UniProt:
  • Canonical: P00691 (Residues: 42-466; Coverage: 67%)
Gene names: BSU03040, amyA, amyE
Sequence domains:
Structure domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: BGC, GLC
Carbohydrate polymer
1 bound ligand:
Ligand CA 3 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-6A
Spacegroup: P212121
Unit cell:
a: 72.59Å b: 74.06Å c: 117.01Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.198 0.198 0.252
Expression system: Bacillus subtilis

Quick links

Citations

3 review citations

Relationship of sequence and structure to specificity in the alpha-amylase family of enzymes.
MacGregor et al. (2001)
2 more

19 mentions without citation

Enzymatic dispersion of biofilms: An emerging biocatalytic avenue to combat biofilm-mediated microbial infections.
Ramakrishnan et al. (2022)
18 more