› 1b9i

X-ray diffraction
2Å resolution

CRYSTAL STRUCTURE OF 3-AMINO-5-HYDROXYBENZOIC ACID (AHBA) SYNTHASE

Released:
DOI: 10.2210/pdb1b9i/pdb
PDB entry 1b9i coloured by chain, front view.
PDB entry 1b9i coloured by chain, side view.
PDB entry 1b9i coloured by chain, top view.
Source organism: Amycolatopsis mediterranei
Primary publication:
Crystal structure of 3-amino-5-hydroxybenzoic acid (AHBA) synthase.
Eads JC, Beeby M, Scapin G, Yu TW, Floss HG
Biochemistry 38 9840-9 (1999)
PMID: 10433690

Function and Biology Details

Reaction catalysed: 
5-amino-5-deoxy-3-dehydroshikimate = 3-amino-5-hydroxybenzoate + H(2)O
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
3-amino-5-hydroxybenzoate synthase Chain: A
Molecule details ›
Chain: A
Length: 388 amino acids
Theoretical weight: 42.25 KDa
Source organism: Amycolatopsis mediterranei
Expression system: Escherichia coli
UniProt:
  • Canonical: O52552 (Residues: 1-388; Coverage: 100%)
Gene names: AMES_0625, RAM_03205, rifD, rifK
Sequence domains: DegT/DnrJ/EryC1/StrS aminotransferase family
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand PXG 1 x PXG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SRS BEAMLINE PX7.2
Spacegroup: P3221
Unit cell:
a: 89.63Å b: 89.63Å c: 126.57Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.225 0.222 0.272
Expression system: Escherichia coli

Quick links

Citations

9 review citations

The manifold of vitamin B6 dependent enzymes.
Schneider et al. (2000)
8 more

1 mention without citation

Structure and mutagenic conversion of E1 dehydrase: at the crossroads of dehydration, amino transfer, and epimerization.
Smith et al. (2008)