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Metal-ion affinity and specificity in EF-hand proteins: coordination geometry and domain plasticity in parvalbumin.

Cates MS, Berry MB, Ho EL, Li Q, Potter JD, Phillips GN
Structure 7 1269-78 (1999)
Related entries: 1b8c, 1b8l, 1b9a

Cited: 56 times
EuropePMC logo PMID: 10545326

Abstract

Background

The EF-hand family is a large set of Ca(2+)-binding proteins that contain characteristic helix-loop-helix binding motifs that are highly conserved in sequence. Members of this family include parvalbumin and many prominent regulatory proteins such as calmodulin and troponin C. EF-hand proteins are involved in a variety of physiological processes including cell-cycle regulation, second messenger production, muscle contraction, microtubule organization and vision.

Results

We have determined the structures of parvalbumin mutants designed to explore the role of the last coordinating residue of the Ca(2+)-binding loop. An E101D substitution has been made in the parvalbumin EF site. The substitution decreases the Ca(2+)-binding affinity 100-fold and increases the Mg(2+)-binding affinity 10-fold. Both the Ca(2+)- and Mg(2+)-bound structures have been determined, and a structural basis has been proposed for the metal-ion-binding properties.

Conclusion

The E101D mutation does not affect the Mg(2+) coordination geometry of the binding loop, but it does pull the F helix 1.1 A towards the loop. The E101D-Ca(2+) structure reveals that this mutant cannot obtain the sevenfold coordination preferred by Ca(2+), presumably because of strain limits imposed by tertiary structure. Analysis of these results relative to previously reported structural information supports a model wherein the characteristics of the last coordinating residue and the plasticity of the Ca(2+)-binding loop delimit the allowable geometries for the coordinating sphere.

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Reviews citing this publication (15)

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  21. Structural implications of Ca2+-dependent actin-bundling function of human EFhd2/Swiprosin-1. Park KR, Kwon MS, An JY, Lee JG, Youn HS, Lee Y, Kang JY, Kim TG, Lim JJ, Park JS, Lee SH, Song WK, Cheong HK, Jun CD, Eom SH. Sci Rep 6 39095 (2016)
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  23. Functional characterization of parvalbumin from the Arctic cod (Boreogadus saida): similarity in calcium affinity among parvalbumins from polar teleosts. Erickson JR, Moerland TS. Comp Biochem Physiol A Mol Integr Physiol 143 228-233 (2006)
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Related citations provided by authors (1)

  1. Restrained Least Squares Refinement of Native (Calcium) and Cadmium- Substituted Carp Parvalbumin Using X-Ray Crystallographic Data at 1.6 Angstrom Resolution. Swain AL, Kretsinger RH, Amma EL J. Biol. Chem. 264 16620- (1989)

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