› 1ax8

X-ray diffraction
2.4Å resolution

Human obesity protein, leptin

Released:
DOI: 10.2210/pdb1ax8/pdb
PDB entry 1ax8 coloured by chain, front view.
PDB entry 1ax8 coloured by chain, side view.
PDB entry 1ax8 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystal structure of the obese protein leptin-E100.
Zhang F, Basinski MB, Beals JM, Briggs SL, Churgay LM, Clawson DK, DiMarchi RD, Furman TC, Hale JE, Hsiung HM, Schoner BE, Smith DP, Zhang XY, Wery JP, Schevitz RW
Nature 387 206-9 (1997)
PMID: 9144295

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo trimer
Assembly name:
PDBe Complex ID:
PDB-CPX-154249 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Leptin Chain: A
Molecule details ›
Chain: A
Length: 146 amino acids
Theoretical weight: 15.98 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P41159 (Residues: 22-167; Coverage: 100%)
Gene names: LEP, OB, OBS
Sequence domains: Leptin
Structure domains: Growth Hormone; Chain: A;

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RUH2R
Spacegroup: P63
Unit cell:
a: 88.14Å b: 88.14Å c: 48.05Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.185 0.185 0.283
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

102 review citations

Leptin.
Ahima et al. (2000)
101 more

38 mentions without citation

How Structural Biologists and the Protein Data Bank Contributed to Recent FDA New Drug Approvals.
Westbrook et al. (2019)
37 more