› 1atp

X-ray diffraction
2.2Å resolution

2.2 angstrom refined crystal structure of the catalytic subunit of cAMP-dependent protein kinase complexed with MNATP and a peptide inhibitor

Released:
DOI: 10.2210/pdb1atp/pdb
PDB entry 1atp coloured by chain, front view.
PDB entry 1atp coloured by chain, side view.
PDB entry 1atp coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
2.2 A refined crystal structure of the catalytic subunit of cAMP-dependent protein kinase complexed with MnATP and a peptide inhibitor.
Zheng J, Trafny EA, Knighton DR, Xuong NH, Taylor SS, Ten Eyck LF, Sowadski JM
Acta Crystallogr D Biol Crystallogr 49 362-5 (1993)
PMID: 15299527

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-138546 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
cAMP-dependent protein kinase catalytic subunit alpha Chain: E
Molecule details ›
Chain: E
Length: 350 amino acids
Theoretical weight: 40.66 KDa
Source organism: Mus musculus
Expression system: Not provided
UniProt:
  • Canonical: P05132 (Residues: 2-351; Coverage: 100%)
Gene names: Pkaca, Prkaca
Sequence domains: Protein kinase domain
Structure domains:
cAMP-dependent protein kinase inhibitor alpha Chain: I
Molecule details ›
Chain: I
Length: 20 amino acids
Theoretical weight: 2.23 KDa
Source organism: Mus musculus
Expression system: Not provided
UniProt:
  • Canonical: P63248 (Residues: 6-25; Coverage: 26%)
Gene name: Pkia

Ligands and Environments

2 bound ligands:
Ligand MN 2 x MN
Ligand ATP 1 x ATP
2 modified residues:
Ligand TPO 1 x TPO
Ligand SEP 1 x SEP

Experiments and Validation Details

Entry percentile scores
Spacegroup: P212121
Unit cell:
a: 73.58Å b: 76.28Å c: 80.58Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.177 0.177 not available
Expression system: Not provided

Quick links

Citations

24 review citations

Mechanisms of specificity in protein phosphorylation.
Ubersax et al. (2007)
23 more

272 mentions without citation

Protein kinases: evolution of dynamic regulatory proteins.
Taylor et al. (2011)
271 more