1at1 Summary

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CRYSTAL STRUCTURES OF PHOSPHONOACETAMIDE LIGATED T AND PHOSPHONOACETAMIDE AND MALONATE LIGATED R STATES OF ASPARTATE CARBAMOYLTRANSFERASE AT 2.8-ANGSTROMS RESOLUTION AND NEUTRAL P*H

The structure was published by Gouaux, J.E. and Lipscomb, W.N., in 1990 in a paper entitled "Crystal structures of phosphonoacetamide ligated T and phosphonoacetamide and malonate ligated R states of aspartate carbamoyltransferase at 2.8-A resolution and neutral pH." (abstract).

This crystal structure was determined using X-ray diffraction at a resolution of 2.8 Å and deposited in 1989.

The experimental data on which the structure is based was not deposited.

This PDB entry contains a complex of 2 biomacromolecules, namely ASPARTATE CARBAMOYLTRANSFERASE, CATALYTIC CHAIN and ASPARTATE CARBAMOYLTRANSFERASE REGULATORY CHAIN.

It also contains one or more heterogenic compounds (e.g., ligands, co-factors, ions, modified amino acids, etc.); see here for a complete list.

The most likely quaternary structure is a 12meric assembly.

The following tables show cross-reference information to other databases (to obtain a list of all PDB entries sharing the same property or classification, click on the magnifying glass icon):


Chain Name UniProt Name of source organism % of UniProt sequence present in the sample Residues in the sample molecules % of residues observed
A ASPARTATE CARBAMOYLTRANSFERASE, CATALYTIC CHAIN P0A786 (2-311) (PYRB_ECOLI)search Escherichia coli K-12search 100% 310 100%
C ASPARTATE CARBAMOYLTRANSFERASE, CATALYTIC CHAIN P0A786 (2-311) (PYRB_ECOLI)search Escherichia coli K-12search 100% 310 100%
B ASPARTATE CARBAMOYLTRANSFERASE REGULATORY CHAIN P0A7F3 (1-153) (PYRI_ECOLI)search Escherichia coli K-12search 95% 153 95%
D ASPARTATE CARBAMOYLTRANSFERASE REGULATORY CHAIN P0A7F3 (1-153) (PYRI_ECOLI)search Escherichia coli K-12search 95% 153 95%


This entry contains 2 unique UniProt proteins:

UniProt accession Name Organism PDB
P0A786 (2 - 311) ASPARTATE CARBAMOYLTRANSFERASE, CATALYTIC CHAIN Escherichia coli
P0A7F3 (1 - 153) ASPARTATE CARBAMOYLTRANSFERASE REGULATORY CHAIN Escherichia coli

Chain Structural classification (SCOP) Structural classification (CATH) Sequence family (Pfam)
A, C (P0A786) Aspartate/ornithine carbamoyltransferasesearch Rossmann foldsearch PF00185: Aspartate/ornithine carbamoyltransferase, Asp/Orn binding domainsearch, PF02729: Aspartate/ornithine carbamoyltransferase, carbamoyl-P binding domainsearch
B, D (P0A7F3) Aspartate carbamoyltransferase, Regulatory-chain, N-terminal domainsearch, Aspartate carbamoyltransferase, Regulatory-chain, C-terminal domainsearch Alpha-Beta Plaitssearch, SH3 type barrels.search PF01948: Aspartate carbamoyltransferase regulatory chain, allosteric domainsearch, PF02748: Aspartate carbamoyltransferase regulatory chain, metal binding domainsearch

Chain ID Molecular function (GO) Biological process (GO) Cellular component (GO)
A, C (P0A786) aspartate carbamoyltransferase activitysearch carboxyl- or carbamoyltransferase activitysearch amino acid bindingsearch protein bindingsearch transferase activitysearch 'de novo' UMP biosynthetic processsearch cellular amino acid metabolic processsearch 'de novo' pyrimidine nucleobase biosynthetic processsearch pyrimidine nucleotide biosynthetic processsearch cytoplasmsearch
B, D (P0A7F3) protein bindingsearch zinc ion bindingsearch metal ion bindingsearch 'de novo' pyrimidine nucleobase biosynthetic processsearch pyrimidine nucleotide biosynthetic processsearch aspartate carbamoyltransferase complexsearch cytoplasmsearch

Chain InterPro annotation
A, C Aspartate carbamoyltransferasesearch Aspartate/ornithine carbamoyltransferasesearch Aspartate/ornithine carbamoyltransferase, Asp/Orn-binding domainsearch Aspartate/ornithine carbamoyltransferase, carbamoyl-P bindingsearch
B, D Aspartate transcarbamylase regulatory subunitsearch Aspartate carbamoyltransferase regulatory subunit, C-terminalsearch Aspartate carbamoyltransferase regulatory subunit, N-terminalsearch