› 1aqu

X-ray diffraction
1.6Å resolution

ESTROGEN SULFOTRANSFERASE WITH BOUND INACTIVE COFACTOR PAP AND 17-BETA ESTRADIOL

Released:
DOI: 10.2210/pdb1aqu/pdb
PDB entry 1aqu coloured by chain, front view.
PDB entry 1aqu coloured by chain, side view.
PDB entry 1aqu coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Crystal structure of estrogen sulphotransferase.
Kakuta Y, Pedersen LG, Carter CW, Negishi M, Pedersen LC
Nat Struct Biol 4 904-8 (1997)
PMID: 9360604

Function and Biology Details

Reaction catalysed: 
3'-phosphoadenylyl sulfate + estrone = adenosine 3',5'-bisphosphate + estrone 3-sulfate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-156073 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Sulfotransferase 1E1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 297 amino acids
Theoretical weight: 35.78 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: P49891 (Residues: 1-295; Coverage: 100%)
Gene names: Est, Ste, Sult1e1
Sequence domains: Sulfotransferase domain
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

2 bound ligands:
Ligand A3P 2 x A3P
Ligand EST 2 x EST
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X9B
Spacegroup: P21212
Unit cell:
a: 97.38Å b: 80.39Å c: 80.95Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.218 0.218 0.251
Expression system: Escherichia coli

Quick links

Citations

25 review citations

Catalytic promiscuity and the evolution of new enzymatic activities.
O'Brien et al. (1999)
24 more

13 mentions without citation

A synergistic approach to protein crystallization: combination of a fixed-arm carrier with surface entropy reduction.
Moon et al. (2010)
12 more