› 1ako

X-ray diffraction
1.7Å resolution

EXONUCLEASE III FROM ESCHERICHIA COLI

Released:
DOI: 10.2210/pdb1ako/pdb
PDB entry 1ako coloured by chain, front view.
PDB entry 1ako coloured by chain, side view.
PDB entry 1ako coloured by chain, top view.
Source organism: Escherichia coli K-12
Primary publication:
Structure and function of the multifunctional DNA-repair enzyme exonuclease III.
Mol CD, Kuo CF, Thayer MM, Cunningham RP, Tainer JA
Nature 374 381-6 (1995)
PMID: 7885481

Function and Biology Details

Reaction catalysed: 
Exonucleolytic cleavage in the 3'- to 5'-direction to yield nucleoside 5'-phosphates
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-140531 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Exodeoxyribonuclease III Chain: A
Molecule details ›
Chain: A
Length: 268 amino acids
Theoretical weight: 31.01 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P09030 (Residues: 1-268; Coverage: 100%)
Gene names: JW1738, b1749, xth, xthA
Sequence domains: Endonuclease/Exonuclease/phosphatase family
Structure domains: Endonuclease/exonuclease/phosphatase

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RUH2R
Spacegroup: P3121
Unit cell:
a: 107.8Å b: 107.8Å c: 42.8Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.169 0.169 0.202
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

42 review citations

Biology of mammalian L1 retrotransposons.
Ostertag et al. (2001)
41 more

36 mentions without citation

Causes of evolutionary rate variation among protein sites.
Echave et al. (2016)
35 more