› 1ais

X-ray diffraction
2.1Å resolution

TATA-BINDING PROTEIN/TRANSCRIPTION FACTOR (II)B/TATA-BOX COMPLEX FROM PYROCOCCUS WOESEI

Released:
DOI: 10.2210/pdb1ais/pdb
PDB entry 1ais coloured by chain, front view.
PDB entry 1ais coloured by chain, side view.
PDB entry 1ais coloured by chain, top view.
Source organism: Pyrococcus woesei
Primary publication:
The 2.1-A crystal structure of an archaeal preinitiation complex: TATA-box-binding protein/transcription factor (II)B core/TATA-box.
Kosa PF, Ghosh G, DeDecker BS, Sigler PB
Proc Natl Acad Sci U S A 94 6042-7 (1997)
PMID: 9177165

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-113424 (preferred)
Entry contents:
2 distinct polypeptide molecules
2 distinct DNA molecules
Macromolecules (4 distinct):
TATA-box-binding protein Chain: A
Molecule details ›
Chain: A
Length: 182 amino acids
Theoretical weight: 20.28 KDa
Source organism: Pyrococcus woesei
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P62001 (Residues: 1-181; Coverage: 95%)
Gene name: tbp
Sequence domains: Transcription factor TFIID (or TATA-binding protein, TBP)
Structure domains: TATA-Binding Protein
Transcription initiation factor IIB Chain: B
Molecule details ›
Chain: B
Length: 200 amino acids
Theoretical weight: 22.59 KDa
Source organism: Pyrococcus woesei
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P61999 (Residues: 101-300; Coverage: 67%)
Gene name: tfb
Sequence domains: Transcription factor TFIIB repeat
Structure domains: Cyclin-like
DNA (5'-D(*AP*AP*CP*TP*TP*AP*CP*TP*TP*TP*(5IU)P*(5IU)P*AP*AP*AP*GP*C)-3') Chain: C
Molecule details ›
Chain: C
Length: 17 nucleotides
Theoretical weight: 5.38 KDa
DNA (5'-D(*GP*CP*TP*TP*TP*AP*AP*AP*AP*AP*GP*TP*AP*AP*GP*TP*T )-3') Chain: E
Molecule details ›
Chain: E
Length: 17 nucleotides
Theoretical weight: 5.25 KDa

Ligands and Environments

No bound ligands
1 modified residue:
Ligand 5IU 2 x 5IU

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE A1
Spacegroup: C2
Unit cell:
a: 125.7Å b: 91.2Å c: 74.2Å
α: 90° β: 122.7° γ: 90°
R-values:
R R work R free
0.212 0.212 0.268
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

16 review citations

Minor groove-binding architectural proteins: structure, function, and DNA recognition.
Bewley et al. (1998)
15 more

21 mentions without citation

New core promoter element in RNA polymerase II-dependent transcription: sequence-specific DNA binding by transcription factor IIB.
Lagrange et al. (1998)
20 more