› 1aih

X-ray diffraction
2.5Å resolution

CATALYTIC DOMAIN OF BACTERIOPHAGE HP1 INTEGRASE

Released:
DOI: 10.2210/pdb1aih/pdb
PDB entry 1aih coloured by chain, front view.
PDB entry 1aih coloured by chain, side view.
PDB entry 1aih coloured by chain, top view.
Source organism: Haemophilus virus HP1
Primary publication:
Molecular organization in site-specific recombination: the catalytic domain of bacteriophage HP1 integrase at 2.7 A resolution.
Hickman AB, Waninger S, Scocca JJ, Dyda F
Cell 89 227-37 (1997)
PMID: 9108478

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-149242 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Integrase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 170 amino acids
Theoretical weight: 19.46 KDa
Source organism: Haemophilus virus HP1
Expression system: Escherichia coli
UniProt:
  • Canonical: P21442 (Residues: 168-337; Coverage: 50%)
Gene name: int
Sequence domains: Phage integrase family
Structure domains: Intergrase catalytic core

Ligands and Environments

2 bound ligands:
Ligand SO4 8 x SO4
Ligand MG 5 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE F2
Spacegroup: P212121
Unit cell:
a: 42.4Å b: 129.3Å c: 234.2Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.209 0.209 0.272
Expression system: Escherichia coli

Quick links

Citations

21 review citations

DNA topoisomerases: structure, function, and mechanism.
Champoux JJ. (2001)
20 more

6 mentions without citation

The structure of an archaeal viral integrase reveals an evolutionarily conserved catalytic core yet supports a mechanism of DNA cleavage in trans.
Eilers et al. (2012)
5 more