› 1afr

X-ray diffraction
2.4Å resolution

STEAROYL-ACYL CARRIER PROTEIN DESATURASE FROM CASTOR SEEDS

Released:
DOI: 10.2210/pdb1afr/pdb
PDB entry 1afr coloured by chain, front view.
PDB entry 1afr coloured by chain, side view.
PDB entry 1afr coloured by chain, top view.
Source organism: Ricinus communis
Primary publication:
Crystal structure of delta9 stearoyl-acyl carrier protein desaturase from castor seed and its relationship to other di-iron proteins.
Lindqvist Y, Huang W, Schneider G, Shanklin J
EMBO J 15 4081-92 (1996)
PMID: 8861937

Function and Biology Details

Reaction catalysed: 
Stearoyl-[acyl-carrier protein] + 2 reduced ferredoxin [iron-sulfur] cluster + O(2) + 2 H(+) = oleoyl-[acyl-carrier protein] + 2 oxidized ferredoxin [iron-sulfur] cluster + 2 H(2)O
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-149597 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Stearoyl-[acyl-carrier-protein] 9-desaturase, chloroplastic Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 345 amino acids
Theoretical weight: 39.75 KDa
Source organism: Ricinus communis
Expression system: Escherichia coli
UniProt:
  • Canonical: P22337 (Residues: 52-396; Coverage: 87%)
Sequence domains: Fatty acid desaturase
Structure domains: Ribonucleotide Reductase, subunit A

Ligands and Environments

1 bound ligand:
Ligand FE2 12 x FE2
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X12C
Spacegroup: P212121
Unit cell:
a: 82.2Å b: 147.8Å c: 198.2Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.22 0.22 0.285
Expression system: Escherichia coli

Quick links

Citations

38 review citations

Biogenesis, molecular regulation and function of plant isoprenoids.
Bouvier et al. (2005)
37 more

15 mentions without citation

Dioxygen Activation by Nonheme Diiron Enzymes: Diverse Dioxygen Adducts, High-Valent Intermediates, and Related Model Complexes.
Jasniewski et al. (2018)
14 more