› 1ac0

Solution NMR

GLUCOAMYLASE, GRANULAR STARCH-BINDING DOMAIN COMPLEX WITH CYCLODEXTRIN, NMR, MINIMIZED AVERAGE STRUCTURE

Released:
DOI: 10.2210/pdb1ac0/pdb
PDB entry 1ac0 coloured by chain, front view.
PDB entry 1ac0 coloured by chain, side view.
PDB entry 1ac0 coloured by chain, top view.
Source organism: Aspergillus niger
Primary publication:
Solution structure of the granular starch binding domain of Aspergillus niger glucoamylase bound to beta-cyclodextrin.
Sorimachi K, Le Gal-Coëffet MF, Williamson G, Archer DB, Williamson MP
Structure 5 647-61 (1997)
PMID: 9195884

Function and Biology Details

Reaction catalysed: 
Hydrolysis of terminal (1->4)-linked alpha-D-glucose residues successively from non-reducing ends of the chains with release of beta-D-glucose
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-159569 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Glucoamylase Chain: A
Molecule details ›
Chain: A
Length: 108 amino acids
Theoretical weight: 11.88 KDa
Source organism: Aspergillus niger
Expression system: Aspergillus niger
UniProt:
  • Canonical: P69328 (Residues: 533-640; Coverage: 17%)
Gene name: GLAA
Sequence domains: Starch binding domain
Structure domains: Immunoglobulins

Ligands and Environments

Carbohydrate polymer : NEW Components: GLC
Carbohydrate polymer
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Refinement method: simulated annealing
Expression system: Aspergillus niger

Quick links

Citations

9 review citations

Carbohydrate-binding domains: multiplicity of biological roles.
Guillén et al. (2010)
8 more

14 mentions without citation

Carbohydrate-binding modules: fine-tuning polysaccharide recognition.
Boraston et al. (2004)
13 more