1a9x Summary

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CARBAMOYL PHOSPHATE SYNTHETASE: CAUGHT IN THE ACT OF GLUTAMINE HYDROLYSIS

The structure was published by Thoden, J.B., Miran, S.G., Phillips, J.C., Howard, A.J., Raushel, F.M., and Holden, H.M., in 1998 in a paper entitled "Carbamoyl phosphate synthetase: caught in the act of glutamine hydrolysis." (abstract).

This crystal structure was determined using X-ray diffraction at a resolution of 1.8 Å and deposited in 1998.

The experimental data on which the structure is based was not deposited.

This PDB entry contains a complex of 2 biomacromolecules, namely CARBAMOYL PHOSPHATE SYNTHETASE (LARGE CHAIN) and CARBAMOYL PHOSPHATE SYNTHETASE (SMALL CHAIN).

It also contains one or more heterogenic compounds (e.g., ligands, co-factors, ions, modified amino acids, etc.); see here for a complete list.

The molecule has more than one probable quaternary state observed. For more details see the quaternary structure page.

The following tables show cross-reference information to other databases (to obtain a list of all PDB entries sharing the same property or classification, click on the magnifying glass icon):


Chain Name UniProt Name of source organism % of UniProt sequence present in the sample Residues in the sample molecules % of residues observed
A CARBAMOYL PHOSPHATE SYNTHETASE (LARGE CHAIN) P00968 (1-1073) (CARB_ECOLI)search Escherichia coli K-12search 100% 1073 98%
C CARBAMOYL PHOSPHATE SYNTHETASE (LARGE CHAIN) P00968 (1-1073) (CARB_ECOLI)search Escherichia coli K-12search 100% 1073 98%
E CARBAMOYL PHOSPHATE SYNTHETASE (LARGE CHAIN) P00968 (1-1073) (CARB_ECOLI)search Escherichia coli K-12search 100% 1073 98%
G CARBAMOYL PHOSPHATE SYNTHETASE (LARGE CHAIN) P00968 (1-1073) (CARB_ECOLI)search Escherichia coli K-12search 100% 1073 98%
B CARBAMOYL PHOSPHATE SYNTHETASE (SMALL CHAIN) P0A6F1 (2-380) (CARA_ECOLI)search Escherichia coli K-12search 99% 379 100%
D CARBAMOYL PHOSPHATE SYNTHETASE (SMALL CHAIN) P0A6F1 (2-380) (CARA_ECOLI)search Escherichia coli K-12search 99% 379 100%
F CARBAMOYL PHOSPHATE SYNTHETASE (SMALL CHAIN) P0A6F1 (2-380) (CARA_ECOLI)search Escherichia coli K-12search 99% 379 100%
H CARBAMOYL PHOSPHATE SYNTHETASE (SMALL CHAIN) P0A6F1 (2-380) (CARA_ECOLI)search Escherichia coli K-12search 99% 379 100%


This entry contains 2 unique UniProt proteins:

UniProt accession Name Organism PDB
P00968 (1 - 1073) CARBAMOYL PHOSPHATE SYNTHETASE (LARGE CHAIN) Escherichia coli
P0A6F1 (2 - 380) CARBAMOYL PHOSPHATE SYNTHETASE (SMALL CHAIN) Escherichia coli

Chain Structural classification (SCOP) Structural classification (CATH) Sequence family (Pfam)
A, C, E, G (P00968) Carbamoyl phosphate synthetase, large subunit connection domainsearch, Carbamoyl phosphate synthetase, large subunit allosteric, C-terminal domainsearch, BC N-terminal domain-likesearch, BC ATP-binding domain-likesearch Rossmann foldsearch, ATP-grasp fold, B domainsearch, ATP-grasp fold, A domainsearch, Carbamoyl Phosphate Synthetase; Chain A, domain 4search PF00289: Carbamoyl-phosphate synthase L chain, N-terminal domainsearch, PF02142: MGS-like domainsearch, PF02786: Carbamoyl-phosphate synthase L chain, ATP binding domainsearch, PF02787: Carbamoyl-phosphate synthetase large chain, oligomerisation domainsearch
B, D, F, H (P0A6F1) Carbamoyl phosphate synthetase, small subunit N-terminal domainsearch, Class I glutamine amidotransferases (GAT)search Carbamoyl phosphate synthetase, small subunit N-terminal domainsearch, Rossmann foldsearch PF00117: Glutamine amidotransferase class-Isearch, PF00988: Carbamoyl-phosphate synthase small chain, CPSase domainsearch

Chain ID Molecular function (GO) Biological process (GO) Cellular component (GO)
A, C, E, G (P00968) ATP bindingsearch metal ion bindingsearch carbamoyl-phosphate synthase (glutamine-hydrolyzing) activitysearch protein bindingsearch nucleotide bindingsearch catalytic activitysearch amino acid bindingsearch ligase activitysearch carbamoyl-phosphate synthase (ammonia) activitysearch cellular amino acid biosynthetic processsearch pyrimidine nucleotide biosynthetic processsearch arginine biosynthetic processsearch 'de novo' UMP biosynthetic processsearch pyrimidine nucleobase biosynthetic processsearch urea cyclesearch biofilm formationsearch metabolic processsearch nitrogen compound metabolic processsearch carbamoyl-phosphate synthase complexsearch
B, D, F, H (P0A6F1) ligase activitysearch protein bindingsearch carbamoyl-phosphate synthase (glutamine-hydrolyzing) activitysearch ATP bindingsearch nucleotide bindingsearch pyrimidine nucleotide biosynthetic processsearch arginine biosynthetic processsearch urea cyclesearch glutamine metabolic processsearch 'de novo' UMP biosynthetic processsearch 'de novo' pyrimidine nucleobase biosynthetic processsearch carbamoyl phosphate biosynthetic processsearch glutamine catabolic processsearch cellular amino acid biosynthetic processsearch cytoplasmsearch cytosolsearch carbamoyl-phosphate synthase complexsearch

Chain InterPro annotation
A, C, E, G Carbamoyl-phosphate synthetase large subunit-like, ATP-binding domainsearch Carbamoyl-phosphate synthetase, large subunit oligomerisation domainsearch Carbamoyl-phosphate synthase, large subunit, N-terminalsearch Carbamoyl-phosphate synthase large subunit, CPSase domainsearch Carbamoyl-phosphate synthase, large subunitsearch Methylglyoxal synthase-like domainsearch ATP-grasp foldsearch ATP-grasp fold, subdomain 1search ATP-grasp fold, subdomain 2search Pre-ATP-grasp domainsearch
B, D, F, H Carbamoyl-phosphate synthase, small subunit N-terminal domainsearch Carbamoyl-phosphate synthase, small subunitsearch Glutamine amidotransferasesearch Class I glutamine amidotransferase-likesearch