› 1a4l

X-ray diffraction
2.6Å resolution

ADA STRUCTURE COMPLEXED WITH DEOXYCOFORMYCIN AT PH 7.0

Released:
DOI: 10.2210/pdb1a4l/pdb
PDB entry 1a4l coloured by chain, front view.
PDB entry 1a4l coloured by chain, side view.
PDB entry 1a4l coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Complexes of adenosine deaminase with two potent inhibitors: X-ray structures in four independent molecules at pH of maximum activity.
Wang Z, Quiocho FA
Biochemistry 37 8314-24 (1998)
PMID: 9622483

Function and Biology Details

Reaction catalysed: 
2'-deoxyadenosine + H(2)O = 2'-deoxyinosine + NH(3)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-137300 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Adenosine deaminase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 349 amino acids
Theoretical weight: 39.72 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: P03958 (Residues: 4-352; Coverage: 99%)
Gene name: Ada
Sequence domains: Adenosine deaminase
Structure domains: Metal-dependent hydrolases

Ligands and Environments

2 bound ligands:
Ligand ZN 4 x ZN
Ligand DCF 4 x DCF
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RUH2R
Spacegroup: P21
Unit cell:
a: 88.929Å b: 93.355Å c: 101.953Å
α: 90° β: 102.94° γ: 90°
R-values:
R R work R free
0.185 0.185 0.272
Expression system: Escherichia coli

Quick links

Citations

5 review citations

Divergent evolution of enzymatic function: mechanistically diverse superfamilies and functionally distinct suprafamilies.
Gerlt et al. (2001)
4 more

9 mentions without citation

Protein design: toward functional metalloenzymes.
Yu et al. (2014)
8 more