› 1a41

X-ray diffraction
2.3Å resolution

TYPE 1-TOPOISOMERASE CATALYTIC FRAGMENT FROM VACCINIA VIRUS

Released:
DOI: 10.2210/pdb1a41/pdb
PDB entry 1a41 coloured by chain, front view.
PDB entry 1a41 coloured by chain, side view.
PDB entry 1a41 coloured by chain, top view.
Source organism: Vaccinia virus
Primary publication:
Conservation of structure and mechanism between eukaryotic topoisomerase I and site-specific recombinases.
Cheng C, Kussie P, Pavletich N, Shuman S
Cell 92 841-50 (1998)
PMID: 9529259

Function and Biology Details

Reaction catalysed: 
ATP-independent breakage of single-stranded DNA, followed by passage and rejoining
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-159498 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DNA topoisomerase 1B Chain: A
Molecule details ›
Chain: A
Length: 234 amino acids
Theoretical weight: 27.27 KDa
Source organism: Vaccinia virus
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P68698 (Residues: 81-314; Coverage: 75%)
Gene names: H6R, OPG111, TOP1, VACWR104
Sequence domains: Eukaryotic DNA topoisomerase I, catalytic core
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand SO4 1 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE A1
Spacegroup: P41212
Unit cell:
a: 81.7Å b: 81.7Å c: 84.5Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.227 0.227 0.309
Expression system: Escherichia coli BL21

Quick links

Citations

32 review citations

DNA topoisomerases: structure, function, and mechanism.
Champoux JJ. (2001)
31 more

4 mentions without citation

Poxvirus proteomics and virus-host protein interactions.
Van Vliet et al. (2009)
3 more