› 1a0j

X-ray diffraction
1.7Å resolution

CRYSTAL STRUCTURE OF A NON-PSYCHROPHILIC TRYPSIN FROM A COLD-ADAPTED FISH SPECIES.

Released:
DOI: 10.2210/pdb1a0j/pdb
PDB entry 1a0j coloured by chain, front view.
PDB entry 1a0j coloured by chain, side view.
PDB entry 1a0j coloured by chain, top view.
Source organism: Salmo salar
Primary publication:
Structure of a non-psychrophilic trypsin from a cold-adapted fish species.
Schrøder HK, Willassen NP, Smalås AO
Acta Crystallogr D Biol Crystallogr 54 780-98 (1998)
PMID: 9757092

Function and Biology Details

Reaction catalysed: 
Preferential cleavage: Arg-|-, Lys-|-.
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-152776 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Trypsin-3 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 223 amino acids
Theoretical weight: 23.8 KDa
Source organism: Salmo salar
UniProt:
  • Canonical: P35033 (Residues: 16-238; Coverage: 97%)
Sequence domains: Trypsin
Structure domains: Trypsin-like serine proteases

Ligands and Environments

3 bound ligands:
Ligand CA 1 x CA
Ligand SO4 10 x SO4
Ligand BEN 4 x BEN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM1A
Spacegroup: P212121
Unit cell:
a: 65.907Å b: 83.107Å c: 154.787Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.173 0.173 0.215

Quick links

Citations

1 review citation

Sustainable Upcycling of Fisheries and Aquaculture Wastes Using Fish-Derived Cold-Adapted Proteases.
Khiari Z. (2022)
8 other articles

24 mentions without citation

Fish trypsins: potential applications in biomedicine and prospects for production.
Jesús-de la Cruz et al. (2018)
23 more