› 1zy7

X-ray diffraction
1.7Å resolution

Crystal structure of the catalytic domain of an adenosine deaminase that acts on RNA (hADAR2) bound to inositol hexakisphosphate (IHP)

Released:
DOI: 10.2210/pdb1zy7/pdb
PDB entry 1zy7 coloured by chain, front view.
PDB entry 1zy7 coloured by chain, side view.
PDB entry 1zy7 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Inositol hexakisphosphate is bound in the ADAR2 core and required for RNA editing.
Macbeth MR, Schubert HL, Vandemark AP, Lingam AT, Hill CP, Bass BL
Science 309 1534-9 (2005)
PMID: 16141067

Function and Biology Details

Reaction catalysed: 
Adenine in double-stranded RNA + H(2)O = hypoxanthine in double-stranded RNA + NH(3)
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-160289 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Double-stranded RNA-specific editase 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 403 amino acids
Theoretical weight: 45.01 KDa
Source organism: Homo sapiens
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: P78563 (Residues: 299-741; Coverage: 54%)
Gene names: ADAR2, ADARB1, DRADA2, RED1
Sequence domains: Adenosine-deaminase (editase) domain

Ligands and Environments

3 bound ligands:
Ligand ZN 2 x ZN
Ligand IHP 2 x IHP
Ligand SO4 1 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P212121
Unit cell:
a: 52.205Å b: 121.192Å c: 127.331Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.175 0.173 0.206
Expression system: Saccharomyces cerevisiae

Quick links

Citations

85 review citations

Functions and regulation of RNA editing by ADAR deaminases.
Nishikura K. (2010)
84 more

18 mentions without citation

New Insights into the Biological Role of Mammalian ADARs; the RNA Editing Proteins.
Mannion et al. (2015)
17 more