› 1zvl

X-ray diffraction
2.5Å resolution

Rat Neuronal Nitric Oxide Synthase Oxygenase Domain complexed with natural substrate L-Arg.

Released:
DOI: 10.2210/pdb1zvl/pdb
PDB entry 1zvl coloured by chain, front view.
PDB entry 1zvl coloured by chain, side view.
PDB entry 1zvl coloured by chain, top view.
Source organism: Rattus norvegicus
Primary publication:
Structural analysis of isoform-specific inhibitors targeting the tetrahydrobiopterin binding site of human nitric oxide synthases.
Matter H, Kumar HS, Fedorov R, Frey A, Kotsonis P, Hartmann E, Fröhlich LG, Reif A, Pfleiderer W, Scheurer P, Ghosh DK, Schlichting I, Schmidt HH
J Med Chem 48 4783-92 (2005)
PMID: 16033258

Function and Biology Details

Reaction catalysed: 
(1a) 2 L-arginine + 2 NADPH + 2 O(2) = 2 N(omega)-hydroxy-L-arginine + 2 NADP(+) + 2 H(2)O
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-151520 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Nitric oxide synthase 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 420 amino acids
Theoretical weight: 48.58 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli
UniProt:
  • Canonical: P29476 (Residues: 298-716; Coverage: 29%)
Gene names: Bnos, Nos1
Sequence domains: Nitric oxide synthase, oxygenase domain
Structure domains:

Ligands and Environments


Cofactor: Ligand HEM 2 x HEM
3 bound ligands:
Ligand ZN 1 x ZN
Ligand H4B 2 x H4B
Ligand ARG 2 x ARG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-2
Spacegroup: P212121
Unit cell:
a: 52.36Å b: 111.289Å c: 165.184Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.203 0.201 0.249
Expression system: Escherichia coli

Quick links

Citations

4 review citations

Target- and mechanism-based therapeutics for neurodegenerative diseases: strength in numbers.
Trippier et al. (2013)
3 more

11 mentions without citation

Development of nitric oxide synthase inhibitors for neurodegeneration and neuropathic pain.
Mukherjee et al. (2014)
10 more