› 1zuw

X-ray diffraction
1.75Å resolution

Crystal structure of B.subtilis glutamate racemase (RacE) with D-Glu

Released:
DOI: 10.2210/pdb1zuw/pdb
PDB entry 1zuw coloured by chain, front view.
PDB entry 1zuw coloured by chain, side view.
PDB entry 1zuw coloured by chain, top view.
Source organism: Bacillus subtilis
Primary publication:
Substrate-induced conformational changes in Bacillus subtilis glutamate racemase and their implications for drug discovery.
Ruzheinikov SN, Taal MA, Sedelnikova SE, Baker PJ, Rice DW
Structure 13 1707-13 (2005)
PMID: 16271894

Function and Biology Details

Reaction catalysed: 
L-glutamate = D-glutamate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-161119 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glutamate racemase 1 Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 272 amino acids
Theoretical weight: 30.03 KDa
Source organism: Bacillus subtilis
Expression system: Escherichia coli
UniProt:
  • Canonical: P94556 (Residues: 1-272; Coverage: 100%)
Gene names: BSU28390, glr, murI, racE
Sequence domains: Asp/Glu/Hydantoin racemase
Structure domains: Rossmann fold

Ligands and Environments

1 bound ligand:
Ligand DGL 3 x DGL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SRS BEAMLINE PX14.1
Spacegroup: C2
Unit cell:
a: 134.12Å b: 60.274Å c: 125.271Å
α: 90° β: 117.34° γ: 90°
R-values:
R R work R free
0.179 0.179 0.237
Expression system: Escherichia coli

Quick links

Citations

4 review citations

Cytoplasmic steps of peptidoglycan biosynthesis.
Barreteau et al. (2008)
3 more

11 mentions without citation

The Blueprint of a Minimal Cell: MiniBacillus.
Reuß et al. (2016)
10 more