› 1ztx

X-ray diffraction
2.5Å resolution

West Nile Virus Envelope Protein DIII in complex with neutralizing E16 antibody Fab

Released:
DOI: 10.2210/pdb1ztx/pdb
PDB entry 1ztx coloured by chain, front view.
PDB entry 1ztx coloured by chain, side view.
PDB entry 1ztx coloured by chain, top view.
Source organisms:
Primary publication:
Structural basis of West Nile virus neutralization by a therapeutic antibody.
Nybakken GE, Oliphant T, Johnson S, Burke S, Diamond MS, Fremont DH
Nature 437 764-9 (2005)
PMID: 16193056

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-212649 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Envelope protein Chain: E
Molecule details ›
Chain: E
Length: 108 amino acids
Theoretical weight: 11.39 KDa
Source organism: West Nile virus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q91KZ4 (Residues: 187-292; Coverage: 36%)
Gene name: E
Sequence domains: Flavivirus glycoprotein, immunoglobulin-like domain
Structure domains: Immunoglobulin-like
Heavy Chain of E16 Antibody Chain: H
Molecule details ›
Chain: H
Length: 219 amino acids
Theoretical weight: 23.36 KDa
Source organism: Mus musculus
Structure domains: Immunoglobulins
Light Chain of E16 Antibody Chain: L
Molecule details ›
Chain: L
Length: 212 amino acids
Theoretical weight: 23.39 KDa
Source organism: Mus musculus
Structure domains: Immunoglobulins

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 4.2.2
Spacegroup: P212121
Unit cell:
a: 52.4Å b: 83.3Å c: 110.6Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.221 0.208 0.282
Expression system: Escherichia coli

Quick links

Citations

58 review citations

Immunity to dengue virus: a tale of original antigenic sin and tropical cytokine storms.
Rothman AL. (2011)
57 more

35 mentions without citation

Focus on flaviviruses: current and future drug targets.
Geiss et al. (2009)
34 more