› 1zp0

X-ray diffraction
3.5Å resolution

Crystal Structure of Mitochondrial Respiratory Complex II bound with 3-nitropropionate and 2-thenoyltrifluoroacetone

Released:
DOI: 10.2210/pdb1zp0/pdb
PDB entry 1zp0 coloured by chain, front view.
PDB entry 1zp0 coloured by chain, side view.
PDB entry 1zp0 coloured by chain, top view.
Source organism: Sus scrofa
Primary publication:
Crystal structure of mitochondrial respiratory membrane protein complex II.
Sun F, Huo X, Zhai Y, Wang A, Xu J, Su D, Bartlam M, Rao Z
Cell 121 1043-57 (2005)
PMID: 15989954

Function and Biology Details

Reaction catalysed: 
Succinate + a quinone = fumarate + a quinol
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
PDBe Complex ID:
PDB-CPX-107594 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
Succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial Chain: A
Molecule details ›
Chain: A
Length: 622 amino acids
Theoretical weight: 68.31 KDa
Source organism: Sus scrofa
UniProt:
  • Canonical: Q0QF01 (Residues: 43-664; Coverage: 94%)
Gene name: SDHA
Sequence domains:
Structure domains:
Succinate dehydrogenase [ubiquinone] iron-sulfur subunit, mitochondrial Chain: B
Molecule details ›
Chain: B
Length: 252 amino acids
Theoretical weight: 28.79 KDa
Source organism: Sus scrofa
UniProt:
  • Canonical: Q007T0 (Residues: 29-279; Coverage: 90%)
Gene name: SDHB
Sequence domains:
Structure domains:
Succinate dehydrogenase cytochrome b560 subunit, mitochondrial Chain: C
Molecule details ›
Chain: C
Length: 140 amino acids
Theoretical weight: 15.3 KDa
Source organism: Sus scrofa
UniProt:
  • Canonical: D0VWV4 (Residues: 30-169; Coverage: 83%)
Gene name: SDHC
Sequence domains: Succinate dehydrogenase/Fumarate reductase transmembrane subunit
Structure domains:
Succinate dehydrogenase [ubiquinone] cytochrome b small subunit, mitochondrial Chain: D
Molecule details ›
Chain: D
Length: 103 amino acids
Theoretical weight: 10.94 KDa
Source organism: Sus scrofa
UniProt:
  • Canonical: A5GZW8 (Residues: 57-159; Coverage: 65%)
Gene name: SDHD
Sequence domains: CybS, succinate dehydrogenase cytochrome B small subunit
Structure domains: Fumarate reductase/succinate dehydrogenase, transmembrane subunit

Ligands and Environments


Cofactor: Ligand HEM 1 x HEM

Cofactor: Ligand FAD 1 x FAD
5 bound ligands:
Ligand 3NP 1 x 3NP
Ligand FES 1 x FES
Ligand SF4 1 x SF4
Ligand F3S 1 x F3S
Ligand TTF 2 x TTF
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P212121
Unit cell:
a: 70.33Å b: 83.531Å c: 294.18Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.302 0.268 0.293

Quick links

Citations

127 review citations

Maturation of iron-sulfur proteins in eukaryotes: mechanisms, connected processes, and diseases.
Lill et al. (2008)
126 more

7 mentions without citation

Cardiac mitochondrial matrix and respiratory complex protein phosphorylation.
Covian et al. (2012)
6 more