› 1ziu

X-ray diffraction
2Å resolution

Crystal Structure of nickel-bound engineered Maltose Binding Protein

Released:
DOI: 10.2210/pdb1ziu/pdb
PDB entry 1ziu coloured by chain, front view.
PDB entry 1ziu coloured by chain, side view.
PDB entry 1ziu coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Structural studies of an engineered zinc biosensor reveal an unanticipated mode of zinc binding.
Telmer PG, Shilton BH
J Mol Biol 354 829-40 (2005)
PMID: 16288781

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-142613 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Maltose/maltodextrin-binding periplasmic protein Chain: A
Molecule details ›
Chain: A
Length: 370 amino acids
Theoretical weight: 40.71 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0AEX9 (Residues: 27-396; Coverage: 100%)
Gene names: JW3994, b4034, malE
Sequence domains: Bacterial extracellular solute-binding protein
Structure domains: Periplasmic binding protein-like II

Ligands and Environments

1 bound ligand:
Ligand NI 2 x NI
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU300
Spacegroup: P1
Unit cell:
a: 37.4Å b: 43.97Å c: 57.88Å
α: 101.15° β: 107.72° γ: 101.9°
R-values:
R R work R free
0.208 0.205 0.243
Expression system: Escherichia coli

Quick links

Citations

4 review citations

Designing artificial enzymes by intuition and computation.
Nanda et al. (2010)
3 more

1 mention without citation

Passenger sequences can promote interlaced dimers in a common variant of the maltose-binding protein.
Momin et al. (2019)