› 1zht

X-ray diffraction
1.9Å resolution

Structure of yeast oxysterol binding protein Osh4 in complex with 7-hydroxycholesterol

Released:
DOI: 10.2210/pdb1zht/pdb
PDB entry 1zht coloured by chain, front view.
PDB entry 1zht coloured by chain, side view.
PDB entry 1zht coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
Structural mechanism for sterol sensing and transport by OSBP-related proteins.
Im YJ, Raychaudhuri S, Prinz WA, Hurley JH
Nature 437 154-8 (2005)
PMID: 16136145

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-153044 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Oxysterol-binding protein homolog 4 Chain: A
Molecule details ›
Chain: A
Length: 438 amino acids
Theoretical weight: 49.9 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P35844 (Residues: 2-434; Coverage: 100%)
Gene names: BSR3, KES1, LPI3C, OSH4, P2614, YPL145C
Sequence domains: Oxysterol-binding protein
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand HCR 1 x HCR
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: C2
Unit cell:
a: 82.316Å b: 95.08Å c: 65.361Å
α: 90° β: 96.55° γ: 90°
R-values:
R R work R free
0.22 0.213 0.239
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

90 review citations

Structure and function of ER membrane contact sites with other organelles.
Phillips et al. (2016)
89 more

5 mentions without citation

Structural mechanism for sterol sensing and transport by OSBP-related proteins.
Im et al. (2005)
4 more