› 1z8x

X-ray diffraction
2Å resolution

Structure of Mutant Pyrrolidone Carboxyl Peptidase (E192V) from a Hyperthermophile, Pyrococcus furiosus

Released:
DOI: 10.2210/pdb1z8x/pdb
PDB entry 1z8x coloured by chain, front view.
PDB entry 1z8x coloured by chain, side view.
PDB entry 1z8x coloured by chain, top view.
Source organism: Pyrococcus furiosus
Primary publication:
Completely buried, non-ion-paired glutamic acid contributes favorably to the conformational stability of pyrrolidone carboxyl peptidases from hyperthermophiles.
Kaushik JK, Iimura S, Ogasahara K, Yamagata Y, Segawa S, Yutani K
Biochemistry 45 7100-12 (2006)
PMID: 16752900

Function and Biology Details

Reaction catalysed: 
Release of an N-terminal pyroglutamyl group from a polypeptide, the second amino acid generally not being Pro.
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-130730 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Pyrrolidone-carboxylate peptidase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 208 amino acids
Theoretical weight: 22.79 KDa
Source organism: Pyrococcus furiosus
Expression system: Escherichia coli
UniProt:
  • Canonical: O73944 (Residues: 1-208; Coverage: 100%)
Gene names: PF1299, pcp
Sequence domains: Pyroglutamyl peptidase
Structure domains: Peptidase C15, pyroglutamyl peptidase I-like

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL40B2
Spacegroup: P21
Unit cell:
a: 56.547Å b: 104.536Å c: 77.363Å
α: 90° β: 92.8° γ: 90°
R-values:
R R work R free
0.22 0.22 0.246
Expression system: Escherichia coli

Quick links

Citations

3 review citations

The role of protonation states in ligand-receptor recognition and binding.
Petukh et al. (2013)
2 more

2 mentions without citation

Rapid catalytic template searching as an enzyme function prediction procedure.
Nilmeier et al. (2013)
1 more