› 1yvj

X-ray diffraction
2.55Å resolution

Crystal structure of the Jak3 kinase domain in complex with a staurosporine analogue

Released:
DOI: 10.2210/pdb1yvj/pdb
PDB entry 1yvj coloured by chain, front view.
PDB entry 1yvj coloured by chain, side view.
PDB entry 1yvj coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystal structure of the Jak3 kinase domain in complex with a staurosporine analog.
Boggon TJ, Li Y, Manley PW, Eck MJ
Blood 106 996-1002 (2005)
PMID: 15831699

Function and Biology Details

Reaction catalysed: 
ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-156548 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Tyrosine-protein kinase JAK3 Chain: A
Molecule details ›
Chain: A
Length: 290 amino acids
Theoretical weight: 33.2 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P52333 (Residues: 814-1103; Coverage: 26%)
Gene name: JAK3
Sequence domains: Protein tyrosine and serine/threonine kinase
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand DTV 2 x DTV
Ligand 4ST 1 x 4ST
1 modified residue:
Ligand PTR 2 x PTR

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU300
Spacegroup: P212121
Unit cell:
a: 46.307Å b: 54.215Å c: 118.607Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.204 0.204 0.254
Expression system: Escherichia coli

Quick links

Citations

25 review citations

The molecular details of cytokine signaling via the JAK/STAT pathway.
Morris et al. (2018)
24 more

29 mentions without citation

JAK3: a two-faced player in hematological disorders.
Cornejo et al. (2009)
28 more