› 1yok

X-ray diffraction
2.5Å resolution

crystal structure of human LRH-1 bound with TIF-2 peptide and phosphatidylglycerol

Released:
DOI: 10.2210/pdb1yok/pdb
PDB entry 1yok coloured by chain, front view.
PDB entry 1yok coloured by chain, side view.
PDB entry 1yok coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural analyses reveal phosphatidyl inositols as ligands for the NR5 orphan receptors SF-1 and LRH-1.
Krylova IN, Sablin EP, Moore J, Xu RX, Waitt GM, MacKay JA, Juzumiene D, Bynum JM, Madauss K, Montana V, Lebedeva L, Suzawa M, Williams JD, Williams SP, Guy RK, Thornton JW, Fletterick RJ, Willson TM, Ingraham HA
Cell 120 343-55 (2005)
PMID: 15707893

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-126311 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Nuclear receptor subfamily 5 group A member 2 Chain: A
Molecule details ›
Chain: A
Length: 256 amino acids
Theoretical weight: 29.8 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: O00482 (Residues: 300-541; Coverage: 45%)
Gene names: B1F, CPF, FTF, NR5A2
Sequence domains: Ligand-binding domain of nuclear hormone receptor
Structure domains: Retinoid X Receptor
Nuclear receptor coactivator 2 Chains: B, C
Molecule details ›
Chains: B, C
Length: 14 amino acids
Theoretical weight: 1.71 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: Q15596 (Residues: 740-753; Coverage: 1%)
Gene names: BHLHE75, NCOA2, SRC2, TIF2

Ligands and Environments

1 bound ligand:
Ligand P6L 1 x P6L
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 17-ID
Spacegroup: P212121
Unit cell:
a: 59.938Å b: 67.199Å c: 79.569Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.23 0.23 0.29
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

78 review citations

Phosphoinositides: tiny lipids with giant impact on cell regulation.
Balla T. (2013)
77 more

10 mentions without citation

Antidiabetic phospholipid-nuclear receptor complex reveals the mechanism for phospholipid-driven gene regulation.
Musille et al. (2012)
9 more