1yfn

X-ray diffraction
1.8Å resolution

Versatile modes of peptide recognition by the AAA+ adaptor protein SspB- the crystal structure of a SspB-RseA complex

Released:

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
PDBe Complex ID:
PDB-CPX-142765 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Stringent starvation protein B Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 118 amino acids
Theoretical weight: 13.17 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0AFZ3 (Residues: 1-118; Coverage: 72%)
Gene names: JW3197, b3228, sspB
Sequence domains: Stringent starvation protein B
Structure domains: SspB-like
Anti-sigma-E factor RseA Chains: E, F, G, H
Molecule details ›
Chains: E, F, G, H
Length: 32 amino acids
Theoretical weight: 3.72 KDa
Source organism: Escherichia coli K-12
Expression system: Not provided
UniProt:
  • Canonical: P0AFX7 (Residues: 77-108; Coverage: 15%)
Gene names: JW2556, b2572, mclA, rseA, yfiJ

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 8-BM
Spacegroup: P21
Unit cell:
a: 69.647Å b: 60.533Å c: 88.576Å
α: 90° β: 94.62° γ: 90°
R-values:
R R work R free
0.241 0.234 0.261
Expression systems:
  • Escherichia coli
  • Not provided