› 1xu7

X-ray diffraction
1.8Å resolution

Crystal Structure of the Interface Open Conformation of Tetrameric 11b-HSD1

Released:
DOI: 10.2210/pdb1xu7/pdb
PDB entry 1xu7 coloured by chain, front view.
PDB entry 1xu7 coloured by chain, side view.
PDB entry 1xu7 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Conformational flexibility in crystal structures of human 11beta-hydroxysteroid dehydrogenase type I provide insights into glucocorticoid interconversion and enzyme regulation.
Hosfield DJ, Wu Y, Skene RJ, Hilgers M, Jennings A, Snell GP, Aertgeerts K
J Biol Chem 280 4639-48 (2005)
PMID: 15513927

Function and Biology Details

Reactions catalysed: 
An 11-beta-hydroxysteroid + NADP(+) = an 11-oxosteroid + NADPH
A 7-beta-hydroxysteroid + NADP(+) = a 7-oxosteroid + NADPH
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assemblies composition:
homo tetramer
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-151298 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
11-beta-hydroxysteroid dehydrogenase 1 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 286 amino acids
Theoretical weight: 31.84 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P28845 (Residues: 24-292; Coverage: 92%)
Gene names: HSD11, HSD11B1, HSD11L, SDR26C1
Sequence domains: short chain dehydrogenase
Structure domains: NAD(P)-binding Rossmann-like Domain

Ligands and Environments


Cofactor: Ligand NDP 4 x NDP
1 bound ligand:
Ligand CPS 6 x CPS
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.3
Spacegroup: P21
Unit cell:
a: 56.43Å b: 159.625Å c: 73.544Å
α: 90° β: 93.07° γ: 90°
R-values:
R R work R free
0.198 0.197 0.217
Expression system: Escherichia coli

Quick links

Citations

13 review citations

11β-hydroxysteroid dehydrogenases: intracellular gate-keepers of tissue glucocorticoid action.
Chapman et al. (2013)
12 more

10 mentions without citation

Crystal structures of 11β-hydroxysteroid dehydrogenase type 1 and their use in drug discovery.
Thomas et al. (2011)
9 more