› 1xrk

X-ray diffraction
1.5Å resolution

Crystal structure of a mutant bleomycin binding protein from Streptoalloteichus hindustanus displaying increased thermostability

Released:
DOI: 10.2210/pdb1xrk/pdb
PDB entry 1xrk coloured by chain, front view.
PDB entry 1xrk coloured by chain, side view.
PDB entry 1xrk coloured by chain, top view.
Source organism: Streptoalloteichus hindustanus
Primary publication:
Engineering a selectable marker for hyperthermophiles.
Brouns SJ, Wu H, Akerboom J, Turnbull AP, de Vos WM, van der Oost J
J Biol Chem 280 11422-31 (2005)
PMID: 15640151

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-148074 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Bleomycin resistance protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 124 amino acids
Theoretical weight: 13.92 KDa
Source organism: Streptoalloteichus hindustanus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P17493 (Residues: 1-124; Coverage: 100%)
Gene name: ble
Sequence domains: Glyoxalase superfamily protein
Structure domains: 2,3-Dihydroxybiphenyl 1,2-Dioxygenase, domain 1

Ligands and Environments

2 bound ligands:
Ligand SO4 3 x SO4
Ligand BLM 2 x BLM
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.2
Spacegroup: P21
Unit cell:
a: 43.983Å b: 66.617Å c: 47.179Å
α: 90° β: 117.43° γ: 90°
R-values:
R R work R free
0.175 0.174 0.194
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

8 review citations

Thermus thermophilus as biological model.
Cava et al. (2009)
7 more

3 mentions without citation

DBAli tools: mining the protein structure space.
Marti-Renom et al. (2007)
2 more