› 1xqf

X-ray diffraction
1.8Å resolution

The mechanism of ammonia transport based on the crystal structure of AmtB of E. coli.

Released:
DOI: 10.2210/pdb1xqf/pdb
PDB entry 1xqf coloured by chain, front view.
PDB entry 1xqf coloured by chain, side view.
PDB entry 1xqf coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
The mechanism of ammonia transport based on the crystal structure of AmtB of Escherichia coli.
Zheng L, Kostrewa D, Bernèche S, Winkler FK, Li XD
Proc Natl Acad Sci U S A 101 17090-5 (2004)
PMID: 15563598

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-159623 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ammonium transporter AmtB Chain: A
Molecule details ›
Chain: A
Length: 418 amino acids
Theoretical weight: 43.62 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P69681 (Residues: 23-428; Coverage: 100%)
Gene names: JW0441, amtB, b0451, ybaG
Sequence domains: Ammonium Transporter Family
Structure domains: Ammonium transporter AmtB like domains

Ligands and Environments

2 bound ligands:
Ligand SO4 2 x SO4
Ligand ACT 1 x ACT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: P63
Unit cell:
a: 111.8Å b: 111.8Å c: 86.4Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.182 0.181 0.208
Expression system: Escherichia coli

Quick links

Citations

45 review citations

Getting across the cell membrane: an overview for small molecules, peptides, and proteins.
Yang et al. (2015)
44 more

12 mentions without citation

The mechanism of ammonia transport based on the crystal structure of AmtB of Escherichia coli.
Zheng et al. (2004)
11 more