› 1xjd

X-ray diffraction
2Å resolution

Crystal Structure of PKC-theta complexed with Staurosporine at 2A resolution

Released:
DOI: 10.2210/pdb1xjd/pdb
PDB entry 1xjd coloured by chain, front view.
PDB entry 1xjd coloured by chain, side view.
PDB entry 1xjd coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Catalytic domain crystal structure of protein kinase C-theta (PKCtheta).
Xu ZB, Chaudhary D, Olland S, Wolfrom S, Czerwinski R, Malakian K, Lin L, Stahl ML, Joseph-McCarthy D, Benander C, Fitz L, Greco R, Somers WS, Mosyak L
J Biol Chem 279 50401-9 (2004)
PMID: 15364937

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-170067 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Protein kinase C theta type Chain: A
Molecule details ›
Chain: A
Length: 345 amino acids
Theoretical weight: 40.82 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q04759 (Residues: 362-706; Coverage: 49%)
Gene names: PRKCQ, PRKCT
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand STU 1 x STU
2 modified residues:
Ligand TPO 1 x TPO
Ligand SEP 1 x SEP

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.2
Spacegroup: C2
Unit cell:
a: 139.566Å b: 42.4Å c: 67.678Å
α: 90° β: 116.24° γ: 90°
R-values:
R R work R free
0.201 0.201 0.216
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

20 review citations

Structural basis of protein kinase C isoform function.
Steinberg SF. (2008)
19 more

30 mentions without citation

Signaling through cAMP and cAMP-dependent protein kinase: diverse strategies for drug design.
Taylor et al. (2008)
29 more