› 1xel

X-ray diffraction
1.8Å resolution

UDP-GALACTOSE 4-EPIMERASE FROM ESCHERICHIA COLI

Released:
DOI: 10.2210/pdb1xel/pdb
PDB entry 1xel coloured by chain, front view.
PDB entry 1xel coloured by chain, side view.
PDB entry 1xel coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Molecular structure of the NADH/UDP-glucose abortive complex of UDP-galactose 4-epimerase from Escherichia coli: implications for the catalytic mechanism.
Thoden JB, Frey PA, Holden HM
Biochemistry 35 5137-44 (1996)
PMID: 8611497

Function and Biology Details

Reaction catalysed: 
UDP-alpha-D-glucose = UDP-alpha-D-galactose
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-140567 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
UDP-glucose 4-epimerase Chain: A
Molecule details ›
Chain: A
Length: 338 amino acids
Theoretical weight: 37.29 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P09147 (Residues: 1-338; Coverage: 100%)
Gene names: JW0742, b0759, galD, galE
Sequence domains: NAD dependent epimerase/dehydratase family
Structure domains:

Ligands and Environments


Cofactor: Ligand NAD 1 x NAD
4 bound ligands:
Ligand NA 2 x NA
Ligand UPG 1 x UPG
Ligand EDO 1 x EDO
Ligand PEG 1 x PEG
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: P3221
Unit cell:
a: 83.8Å b: 83.8Å c: 108.4Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.137 not available not available
Expression system: Escherichia coli

Quick links

Citations

7 review citations

Unusual sugar biosynthesis and natural product glycodiversification.
Thibodeaux et al. (2007)
6 more

14 mentions without citation

Medium- and short-chain dehydrogenase/reductase gene and protein families : the SDR superfamily: functional and structural diversity within a family of metabolic and regulatory enzymes.
Kavanagh et al. (2008)
13 more