› 1xdy

X-ray diffraction
2.2Å resolution

Structural and Biochemical Identification of a Novel Bacterial Oxidoreductase, W-containing cofactor

Released:
DOI: 10.2210/pdb1xdy/pdb
PDB entry 1xdy coloured by chain, front view.
PDB entry 1xdy coloured by chain, side view.
PDB entry 1xdy coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Structural and biochemical identification of a novel bacterial oxidoreductase.
Loschi L, Brokx SJ, Hills TL, Zhang G, Bertero MG, Lovering AL, Weiner JH, Strynadka NC
J Biol Chem 279 50391-400 (2004)
PMID: 15355966

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-160080 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Protein-methionine-sulfoxide reductase catalytic subunit MsrP Chains: A, B, C, D, E, F, G, H, I, J
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J
Length: 298 amino acids
Theoretical weight: 33.67 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P76342 (Residues: 45-334; Coverage: 100%)
Gene names: JW1954, b1971, msrP, yedY
Sequence domains: Oxidoreductase molybdopterin binding domain
Structure domains: Oxidoreductase, molybdopterin-binding domain

Ligands and Environments

2 bound ligands:
Ligand W 10 x W
Ligand MTE 10 x MTE
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.2
Spacegroup: P21
Unit cell:
a: 84.842Å b: 177.513Å c: 104.214Å
α: 90° β: 109.73° γ: 90°
R-values:
R R work R free
0.226 0.226 0.258
Expression system: Escherichia coli

Quick links

Citations

16 review citations

Oxidative stress, protein damage and repair in bacteria.
Ezraty et al. (2017)
15 more

1 mention without citation

Mining the Flavoproteome of Brucella ovis, the Brucellosis Causing Agent in Ovis aries.
Minjárez-Sáenz et al. (2022)