› 1x8o

X-ray diffraction
1.01Å resolution

1.01 A Crystal Structure Of Nitrophorin 4 From Rhodnius Prolixus Complexed With Nitric Oxide at pH 5.6

Released:
DOI: 10.2210/pdb1x8o/pdb
PDB entry 1x8o coloured by chain, front view.
PDB entry 1x8o coloured by chain, side view.
PDB entry 1x8o coloured by chain, top view.
Source organism: Rhodnius prolixus
Primary publication:
Protein functional cycle viewed at atomic resolution: conformational change and mobility in nitrophorin 4 as a function of pH and NO binding.
Kondrashov DA, Roberts SA, Weichsel A, Montfort WR
Biochemistry 43 13637-47 (2004)
PMID: 15504026

Function and Biology Details

Reaction catalysed: 
3 nitrite + 2 H(+) = 2 nitric oxide + nitrate + H(2)O
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-188198 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Nitrophorin-4 Chain: A
Molecule details ›
Chain: A
Length: 184 amino acids
Theoretical weight: 20.29 KDa
Source organism: Rhodnius prolixus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q94734 (Residues: 22-205; Coverage: 100%)
Sequence domains: Nitrophorin
Structure domains: Lipocalin

Ligands and Environments


Cofactor: Ligand HEM 1 x HEM
2 bound ligands:
Ligand PO4 1 x PO4
Ligand NO 1 x NO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-1
Spacegroup: C2
Unit cell:
a: 70.445Å b: 42.729Å c: 53.098Å
α: 90° β: 94.43° γ: 90°
R-values:
R R work R free
0.103 0.102 0.13
Expression system: Escherichia coli BL21(DE3)

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Citations

2 review citations

Nitrophorins: nitrite disproportionation reaction and other novel functionalities of insect heme-based nitric oxide transport proteins.
Knipp et al. (2011)
1 more

8 mentions without citation

pH-Dependent conformational changes in proteins and their effect on experimental pK(a)s: the case of Nitrophorin 4.
Di Russo et al. (2012)
7 more