› 1x7p

X-ray diffraction
2.55Å resolution

Crystal structure of the SpoU Methyltransferase AviRb from Streptomyces viridochromogenes in complex with the cofactor AdoMet

Released:
DOI: 10.2210/pdb1x7p/pdb
PDB entry 1x7p coloured by chain, front view.
PDB entry 1x7p coloured by chain, side view.
PDB entry 1x7p coloured by chain, top view.
Source organism: Streptomyces viridochromogenes
Primary publication:
Structure and function of the antibiotic resistance-mediating methyltransferase AviRb from Streptomyces viridochromogenes.
Mosbacher TG, Bechthold A, Schulz GE
J Mol Biol 345 535-45 (2005)
PMID: 15581897

Function and Biology Details

Reaction catalysed: 
S-adenosyl-L-methionine + uridine(2479) in 23S rRNA = S-adenosyl-L-homocysteine + 2'-O-methyluridine(2479) in 23S rRNA
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-190256 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
23S rRNA (uridine(2479)-2'-O)-methyltransferase Chains: A, B
Molecule details ›
Chains: A, B
Length: 287 amino acids
Theoretical weight: 31.25 KDa
Source organism: Streptomyces viridochromogenes
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9F5K6 (Residues: 1-287; Coverage: 100%)
Gene name: aviRb
Sequence domains: SpoU rRNA Methylase family
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand SAM 2 x SAM
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: P41212
Unit cell:
a: 76.881Å b: 76.881Å c: 209.818Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.205 0.202 0.257
Expression system: Escherichia coli BL21(DE3)

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Citations

1 review citation

How does a knotted protein fold?
Mallam AL. (2009)
27 other articles

4 mentions without citation

Tied up in knots: Untangling substrate recognition by the SPOUT methyltransferases.
Strassler et al. (2022)
3 more