› 1x1n

X-ray diffraction
1.8Å resolution

Structure determination and refinement at 1.8 A resolution of Disproportionating Enzyme from Potato

Released:
DOI: 10.2210/pdb1x1n/pdb
PDB entry 1x1n coloured by chain, front view.
PDB entry 1x1n coloured by chain, side view.
PDB entry 1x1n coloured by chain, top view.
Source organism: Solanum tuberosum
Entry authors: Imamura K, Matsuura T, Takaha T, Fujii K, Nakagawa A, Kusunoki M, Nitta Y

Function and Biology Details

Reaction catalysed: 
Transfers a segment of a (1->4)-alpha-D-glucan to a new position in an acceptor, which may be glucose or a (1->4)-alpha-D-glucan
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-170395 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
4-alpha-glucanotransferase, chloroplastic/amyloplastic Chain: A
Molecule details ›
Chain: A
Length: 524 amino acids
Theoretical weight: 59.22 KDa
Source organism: Solanum tuberosum
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q06801 (Residues: 53-576; Coverage: 91%)
Gene name: DPEP
Sequence domains: 4-alpha-glucanotransferase
Structure domains: Glycosidases

Ligands and Environments

2 bound ligands:
Ligand CA 1 x CA
Ligand GOL 9 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE AR-NW12A
Spacegroup: C2221
Unit cell:
a: 69.869Å b: 120.535Å c: 174.382Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.19 0.179 0.21
Expression system: Escherichia coli BL21(DE3)

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