› 1wzl

X-ray diffraction
2Å resolution

Thermoactinomyces vulgaris R-47 alpha-amylase II (TVA II) mutatnt R469L

Released:
DOI: 10.2210/pdb1wzl/pdb
PDB entry 1wzl coloured by chain, front view.
PDB entry 1wzl coloured by chain, side view.
PDB entry 1wzl coloured by chain, top view.
Source organism: Thermoactinomyces vulgaris
Entry authors: Mizuno M, Ichikawa K, Tonozuka T, Ohtaki A, Shimura Y, Kamitori S, Nishikawa A, Sakano Y

Function and Biology Details

Reaction catalysed: 
Hydrolysis of pullulan to panose (6-alpha-D-glucosylmaltose)
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-170689 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Neopullulanase 2 Chains: A, B
Molecule details ›
Chains: A, B
Length: 585 amino acids
Theoretical weight: 67.51 KDa
Source organism: Thermoactinomyces vulgaris
Expression system: Escherichia coli
UniProt:
  • Canonical: Q08751 (Residues: 1-585; Coverage: 100%)
Gene name: tvaII
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand CA 2 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-18B
Spacegroup: P212121
Unit cell:
a: 112.874Å b: 117.907Å c: 113.142Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.195 0.195 0.229
Expression system: Escherichia coli

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