› 1wta

X-ray diffraction
1.78Å resolution

Crystal Structure of 5'-Deoxy-5'-methylthioadenosine from Aeropyrum pernix (R32 form)

Released:
DOI: 10.2210/pdb1wta/pdb
PDB entry 1wta coloured by chain, front view.
PDB entry 1wta coloured by chain, side view.
PDB entry 1wta coloured by chain, top view.
Source organism: Aeropyrum pernix K1
Entry authors: Tsunoda M, Murakami Y, Nakamura KT

Function and Biology Details

Reaction catalysed: 
S-methyl-5'-thioadenosine + phosphate = adenine + S-methyl-5-thio-alpha-D-ribose 1-phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
homo trimer (preferred)
homo hexamer
Assembly name:
PDBe Complex ID:
PDB-CPX-195442 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
S-methyl-5'-thioadenosine phosphorylase Chain: A
Molecule details ›
Chain: A
Length: 275 amino acids
Theoretical weight: 30.78 KDa
Source organism: Aeropyrum pernix K1
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9YAQ8 (Residues: 1-275; Coverage: 100%)
Gene names: APE_1885, mtnP
Sequence domains: Phosphorylase superfamily
Structure domains: Nucleoside phosphorylase domain

Ligands and Environments

2 bound ligands:
Ligand PO4 1 x PO4
Ligand ADE 1 x ADE
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU300
Spacegroup: R32
Unit cell:
a: 78.15Å b: 78.15Å c: 230.43Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.166 0.164 0.196
Expression system: Escherichia coli

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