› 1wke

X-ray diffraction
2.2Å resolution

TRNA-GUANINE TRANSGLYCOSYLASE

Released:
DOI: 10.2210/pdb1wke/pdb
PDB entry 1wke coloured by chain, front view.
PDB entry 1wke coloured by chain, side view.
PDB entry 1wke coloured by chain, top view.
Source organism: Zymomonas mobilis
Primary publication:
Mutagenesis and crystallographic studies of Zymomonas mobilis tRNA-guanine transglycosylase reveal aspartate 102 as the active site nucleophile.
Romier C, Reuter K, Suck D, Ficner R
Biochemistry 35 15734-9 (1996)
PMID: 8961936

Function and Biology Details

Reaction catalysed: 
Guanine(34) in tRNA + 7-aminomethyl-7-carbaguanine = 7-aminomethyl-7-carbaguanine(34) in tRNA + guanine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-151272 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Queuine tRNA-ribosyltransferase Chain: A
Molecule details ›
Chain: A
Length: 386 amino acids
Theoretical weight: 42.88 KDa
Source organism: Zymomonas mobilis
Expression system: Escherichia coli
UniProt:
  • Canonical: P28720 (Residues: 1-386; Coverage: 100%)
Gene names: ZMO0363, tgt
Sequence domains: Queuine tRNA-ribosyltransferase
Structure domains: Queuine tRNA-ribosyltransferase-like

Ligands and Environments

1 bound ligand:
Ligand ZN 1 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: C2
Unit cell:
a: 90.2Å b: 64.5Å c: 72Å
α: 90° β: 96.9° γ: 90°
R-values:
R R work R free
0.203 0.203 0.246
Expression system: Escherichia coli

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Citations

11 review citations

Biosynthesis of the 7-deazaguanosine hypermodified nucleosides of transfer RNA.
Iwata-Reuyl D. (2003)
10 more