1wej

X-ray diffraction
1.8Å resolution

IGG1 FAB FRAGMENT (OF E8 ANTIBODY) COMPLEXED WITH HORSE CYTOCHROME C AT 1.8 A RESOLUTION

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-224909 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Immunoglobulin kappa chain variable 12-41 Chain: L
Molecule details ›
Chain: L
Length: 214 amino acids
Theoretical weight: 23.66 KDa
Source organism: Mus musculus
UniProt:
  • Canonical: P01635 (Residues: 21-115; Coverage: 100%)
Gene names: Gm16848, Igkv12-41
Sequence domains: Immunoglobulin V-set domain
Structure domains: Immunoglobulins
E8 ANTIBODY Chain: H
Molecule details ›
Chain: H
Length: 223 amino acids
Theoretical weight: 23.85 KDa
Source organism: Mus musculus
Structure domains: Immunoglobulins
Cytochrome c Chain: F
Molecule details ›
Chain: F
Length: 105 amino acids
Theoretical weight: 11.75 KDa
Source organism: Equus caballus
UniProt:
  • Canonical: P00004 (Residues: 2-105; Coverage: 99%)
Gene names: CYC, CYCS
Sequence domains: Cytochrome c
Structure domains: Cytochrome c-like domain

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL7-1
Spacegroup: P212121
Unit cell:
a: 79.58Å b: 72.54Å c: 94.38Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.2 0.2 0.256