› 1wcg

X-ray diffraction
1.1Å resolution

Function and Biology Details

Reaction catalysed: 
A thioglucoside + H(2)O = a sugar + a thiol
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-188279 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Myrosinase 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 464 amino acids
Theoretical weight: 53.8 KDa
Source organism: Brevicoryne brassicae
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q95X01 (Residues: 1-464; Coverage: 100%)
Sequence domains: Glycosyl hydrolase family 1
Structure domains: Glycosidases

Ligands and Environments

1 bound ligand:
Ligand GOL 5 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-2
Spacegroup: P21
Unit cell:
a: 73.853Å b: 67.365Å c: 97.301Å
α: 90° β: 93.7° γ: 90°
R-values:
R R work R free
0.133 0.132 0.146
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 review citation

Gut Glucosinolate Metabolism and Isothiocyanate Production.
Narbad et al. (2018)
24 other articles

5 mentions without citation

High resolution crystal structure of human β-glucuronidase reveals structural basis of lysosome targeting.
Hassan et al. (2013)
4 more