› 1w77

X-ray diffraction
2Å resolution

Function and Biology Details

Reaction catalysed: 
CTP + 2-C-methyl-D-erythritol 4-phosphate = diphosphate + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-159673 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase, chloroplastic Chain: A
Molecule details ›
Chain: A
Length: 228 amino acids
Theoretical weight: 25.5 KDa
Source organism: Arabidopsis thaliana
Expression system: Escherichia coli
UniProt:
  • Canonical: P69834 (Residues: 75-302; Coverage: 76%)
Gene names: At2g02500, ISPD, MCT, MECT, MEPCT, T8K22.20
Sequence domains: 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase
Structure domains: Spore Coat Polysaccharide Biosynthesis Protein SpsA; Chain A

Ligands and Environments

3 bound ligands:
Ligand CD 1 x CD
Ligand CU 4 x CU
Ligand C5P 1 x C5P
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4
Spacegroup: R32
Unit cell:
a: 74.496Å b: 74.496Å c: 223.026Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.237 0.232 0.349
Expression system: Escherichia coli

Quick links

Citations

5 review citations

Mechanistic aspects of carotenoid biosynthesis.
Moise et al. (2014)
4 more

3 mentions without citation

New Insight into Isoprenoids Biosynthesis Process and Future Prospects for Drug Designing in Plasmodium.
Saggu et al. (2016)
2 more