› 1w4x

X-ray diffraction
1.7Å resolution

Phenylacetone Monooxygenase, a Baeyer-Villiger Monooxygenase

Released:
DOI: 10.2210/pdb1w4x/pdb
PDB entry 1w4x coloured by chain, front view.
PDB entry 1w4x coloured by chain, side view.
PDB entry 1w4x coloured by chain, top view.
Source organism: Thermobifida fusca
Primary publication:
Crystal structure of a Baeyer-Villiger monooxygenase.
Malito E, Alfieri A, Fraaije MW, Mattevi A
Proc Natl Acad Sci U S A 101 13157-62 (2004)
PMID: 15328411

Function and Biology Details

Reaction catalysed: 
Phenylacetone + NADPH + O(2) = benzyl acetate + NADP(+) + H(2)O
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-175303 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Phenylacetone monooxygenase Chain: A
Molecule details ›
Chain: A
Length: 542 amino acids
Theoretical weight: 61.19 KDa
Source organism: Thermobifida fusca
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q47PU3 (Residues: 1-542; Coverage: 100%)
Gene names: Tfu_1490, pamO
Sequence domains: Flavin-binding monooxygenase-like
Structure domains: FAD/NAD(P)-binding domain

Ligands and Environments


Cofactor: Ligand FAD 1 x FAD
1 bound ligand:
Ligand SO4 1 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-1
Spacegroup: I222
Unit cell:
a: 86.472Å b: 115.652Å c: 165.254Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.213 0.212 0.244
Expression system: Escherichia coli BL21

Quick links

Citations

18 review citations

Flavoprotein monooxygenases, a diverse class of oxidative biocatalysts.
van Berkel et al. (2006)
17 more

21 mentions without citation

Engineering non-heme mono- and dioxygenases for biocatalysis.
Dror et al. (2012)
20 more