› 1w23

X-ray diffraction
1.08Å resolution

Crystal structure of phosphoserine aminotransferase from Bacillus alcalophilus

Released:
DOI: 10.2210/pdb1w23/pdb
PDB entry 1w23 coloured by chain, front view.
PDB entry 1w23 coloured by chain, side view.
PDB entry 1w23 coloured by chain, top view.
Source organism: Alkalihalobacillus alcalophilus
Primary publication:
Enzyme adaptation to alkaline pH: atomic resolution (1.08 A) structure of phosphoserine aminotransferase from Bacillus alcalophilus.
Dubnovitsky AP, Kapetaniou EG, Papageorgiou AC
Protein Sci 14 97-110 (2005)
PMID: 15608117

Function and Biology Details

Reaction catalysed: 
4-phosphonooxy-L-threonine + 2-oxoglutarate = (3R)-3-hydroxy-2-oxo-4-phosphonooxybutanoate + L-glutamate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-193203 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Phosphoserine aminotransferase Chains: A, B
Molecule details ›
Chains: A, B
Length: 360 amino acids
Theoretical weight: 40.24 KDa
Source organism: Alkalihalobacillus alcalophilus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9RME2 (Residues: 2-361; Coverage: 100%)
Gene name: serC
Sequence domains: Aminotransferase class-V
Structure domains:

Ligands and Environments


Cofactor: Ligand PLP 2 x PLP
6 bound ligands:
Ligand MG 4 x MG
Ligand PEG 1 x PEG
Ligand PGE 3 x PGE
Ligand CL 4 x CL
Ligand GOL 1 x GOL
Ligand EPE 1 x EPE
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE BW7A
Spacegroup: P21212
Unit cell:
a: 144.467Å b: 84.84Å c: 67.469Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.117 not available 0.139
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

4 review citations

Alkaline pH homeostasis in bacteria: new insights.
Padan et al. (2005)
3 more

7 mentions without citation

The Blueprint of a Minimal Cell: MiniBacillus.
Reuß et al. (2016)
6 more